Bio-isoprene production using exogenous MVA pathway and isoprene synthase in Escherichia coli.
Identifieur interne : 002B86 ( Main/Exploration ); précédent : 002B85; suivant : 002B87Bio-isoprene production using exogenous MVA pathway and isoprene synthase in Escherichia coli.
Auteurs : Jianming Yang [République populaire de Chine] ; Guang Zhao ; Yuanzhang Sun ; Yanning Zheng ; Xinglin Jiang ; Wei Liu ; Mo XianSource :
- Bioresource technology [ 1873-2976 ] ; 2012.
Descripteurs français
- KwdFr :
- Alkyl et aryl transferases (génétique), Alkyl et aryl transferases (métabolisme), Amélioration génétique (méthodes), Butadiènes (isolement et purification), Clonage moléculaire (MeSH), Escherichia coli (enzymologie), Escherichia coli (génétique), Hémiterpènes (biosynthèse), Hémiterpènes (isolement et purification), Pentanes (isolement et purification), Protéines recombinantes (métabolisme), Saccharomyces cerevisiae (génétique), Saccharomyces cerevisiae (métabolisme), Transduction du signal (physiologie).
- MESH :
- biosynthèse : Hémiterpènes.
- enzymologie : Escherichia coli.
- génétique : Alkyl et aryl transferases, Escherichia coli, Saccharomyces cerevisiae.
- isolement et purification : Butadiènes, Hémiterpènes, Pentanes.
- métabolisme : Alkyl et aryl transferases, Protéines recombinantes, Saccharomyces cerevisiae.
- méthodes : Amélioration génétique.
- physiologie : Transduction du signal.
- Clonage moléculaire.
English descriptors
- KwdEn :
- Alkyl and Aryl Transferases (genetics), Alkyl and Aryl Transferases (metabolism), Butadienes (isolation & purification), Cloning, Molecular (MeSH), Escherichia coli (enzymology), Escherichia coli (genetics), Genetic Enhancement (methods), Hemiterpenes (biosynthesis), Hemiterpenes (isolation & purification), Pentanes (isolation & purification), Recombinant Proteins (metabolism), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (metabolism), Signal Transduction (physiology).
- MESH :
- chemical , biosynthesis : Hemiterpenes.
- chemical , genetics : Alkyl and Aryl Transferases.
- chemical , isolation & purification : Butadienes, Hemiterpenes, Pentanes.
- chemical , metabolism : Alkyl and Aryl Transferases, Recombinant Proteins.
- enzymology : Escherichia coli.
- genetics : Escherichia coli, Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- methods : Genetic Enhancement.
- physiology : Signal Transduction.
- Cloning, Molecular.
Abstract
In this paper, an original strategy is employed to biosynthesize the isoprene by heterologously co-expressing the Saccharomyces cerevisiae MVA pathway and isoprene synthase (IspS) from Populus alba in the Escherichia coli BL21 (DE3) strain, which was screened from three different IspS enzymes. The finally genetic strain YJM13 harboring the MVA pathway and ispS(Pa) gene could accumulate isoprene up to 2.48 mg/l and 532 mg/l under the flask and fed-batch fermentation conditions, respectively, which is about three times and five times to the control strain. The result proves to be higher than that in the report documents. In this way, a potential production system for isoprene from renewable sources via the MVA pathway in E. coli has been provided.
DOI: 10.1016/j.biortech.2011.10.042
PubMed: 22133602
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Sun</name></author><author><name sortKey="Zheng, Yanning" sort="Zheng, Yanning" uniqKey="Zheng Y" first="Yanning" last="Zheng">Yanning Zheng</name></author><author><name sortKey="Jiang, Xinglin" sort="Jiang, Xinglin" uniqKey="Jiang X" first="Xinglin" last="Jiang">Xinglin Jiang</name></author><author><name sortKey="Liu, Wei" sort="Liu, Wei" uniqKey="Liu W" first="Wei" last="Liu">Wei Liu</name></author><author><name sortKey="Xian, Mo" sort="Xian, Mo" uniqKey="Xian M" first="Mo" last="Xian">Mo Xian</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22133602</idno><idno type="pmid">22133602</idno><idno type="doi">10.1016/j.biortech.2011.10.042</idno><idno type="wicri:Area/Main/Corpus">002C11</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002C11</idno><idno type="wicri:Area/Main/Curation">002C11</idno><idno type="wicri:explorRef" wicri:stream="Main" 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sort="Sun, Yuanzhang" uniqKey="Sun Y" first="Yuanzhang" last="Sun">Yuanzhang Sun</name></author><author><name sortKey="Zheng, Yanning" sort="Zheng, Yanning" uniqKey="Zheng Y" first="Yanning" last="Zheng">Yanning Zheng</name></author><author><name sortKey="Jiang, Xinglin" sort="Jiang, Xinglin" uniqKey="Jiang X" first="Xinglin" last="Jiang">Xinglin Jiang</name></author><author><name sortKey="Liu, Wei" sort="Liu, Wei" uniqKey="Liu W" first="Wei" last="Liu">Wei Liu</name></author><author><name sortKey="Xian, Mo" sort="Xian, Mo" uniqKey="Xian M" first="Mo" last="Xian">Mo Xian</name></author></analytic><series><title level="j">Bioresource technology</title><idno type="eISSN">1873-2976</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkyl and Aryl Transferases (genetics)</term><term>Alkyl and Aryl Transferases (metabolism)</term><term>Butadienes (isolation & purification)</term><term>Cloning, Molecular (MeSH)</term><term>Escherichia coli (enzymology)</term><term>Escherichia coli (genetics)</term><term>Genetic Enhancement (methods)</term><term>Hemiterpenes (biosynthesis)</term><term>Hemiterpenes (isolation & purification)</term><term>Pentanes (isolation & purification)</term><term>Recombinant Proteins (metabolism)</term><term>Saccharomyces cerevisiae (genetics)</term><term>Saccharomyces cerevisiae (metabolism)</term><term>Signal Transduction (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alkyl et aryl transferases (génétique)</term><term>Alkyl et aryl transferases (métabolisme)</term><term>Amélioration génétique (méthodes)</term><term>Butadiènes (isolement et purification)</term><term>Clonage moléculaire (MeSH)</term><term>Escherichia coli (enzymologie)</term><term>Escherichia coli (génétique)</term><term>Hémiterpènes (biosynthèse)</term><term>Hémiterpènes (isolement et purification)</term><term>Pentanes (isolement et purification)</term><term>Protéines recombinantes (métabolisme)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Saccharomyces cerevisiae (métabolisme)</term><term>Transduction du signal (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Hemiterpenes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Alkyl and Aryl Transferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Butadienes</term><term>Hemiterpenes</term><term>Pentanes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Alkyl and Aryl Transferases</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Hémiterpènes</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Alkyl et aryl transferases</term><term>Escherichia coli</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Butadiènes</term><term>Hémiterpènes</term><term>Pentanes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetic Enhancement</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Alkyl et aryl transferases</term><term>Protéines recombinantes</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Amélioration génétique</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Transduction du signal</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Signal Transduction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this paper, an original strategy is employed to biosynthesize the isoprene by heterologously co-expressing the Saccharomyces cerevisiae MVA pathway and isoprene synthase (IspS) from Populus alba in the Escherichia coli BL21 (DE3) strain, which was screened from three different IspS enzymes. The finally genetic strain YJM13 harboring the MVA pathway and ispS(Pa) gene could accumulate isoprene up to 2.48 mg/l and 532 mg/l under the flask and fed-batch fermentation conditions, respectively, which is about three times and five times to the control strain. The result proves to be higher than that in the report documents. In this way, a potential production system for isoprene from renewable sources via the MVA pathway in E. coli has been provided.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22133602</PMID><DateCompleted><Year>2012</Year><Month>04</Month><Day>18</Day></DateCompleted><DateRevised><Year>2012</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2976</ISSN><JournalIssue CitedMedium="Internet"><Volume>104</Volume><PubDate><Year>2012</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Bioresource technology</Title><ISOAbbreviation>Bioresour Technol</ISOAbbreviation></Journal><ArticleTitle>Bio-isoprene production using exogenous MVA pathway and isoprene synthase in Escherichia coli.</ArticleTitle><Pagination><MedlinePgn>642-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biortech.2011.10.042</ELocationID><Abstract><AbstractText>In this paper, an original strategy is employed to biosynthesize the isoprene by heterologously co-expressing the Saccharomyces cerevisiae MVA pathway and isoprene synthase (IspS) from Populus alba in the Escherichia coli BL21 (DE3) strain, which was screened from three different IspS enzymes. The finally genetic strain YJM13 harboring the MVA pathway and ispS(Pa) gene could accumulate isoprene up to 2.48 mg/l and 532 mg/l under the flask and fed-batch fermentation conditions, respectively, which is about three times and five times to the control strain. The result proves to be higher than that in the report documents. In this way, a potential production system for isoprene from renewable sources via the MVA pathway in E. coli has been provided.</AbstractText><CopyrightInformation>Copyright © 2011 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Jianming</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Guang</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Yuanzhang</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Yanning</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Xinglin</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Xian</LastName><ForeName>Mo</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>10</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Bioresour Technol</MedlineTA><NlmUniqueID>9889523</NlmUniqueID><ISSNLinking>0960-8524</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002070">Butadienes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045782">Hemiterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010420">Pentanes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0A62964IBU</RegistryNumber><NameOfSubstance UI="C005059">isoprene</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.-</RegistryNumber><NameOfSubstance UI="D019883">Alkyl and Aryl Transferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.1.-</RegistryNumber><NameOfSubstance UI="C093854">isoprene synthase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019883" MajorTopicYN="N">Alkyl and Aryl Transferases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002070" MajorTopicYN="N">Butadienes</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D024861" MajorTopicYN="N">Genetic Enhancement</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045782" MajorTopicYN="N">Hemiterpenes</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010420" MajorTopicYN="N">Pentanes</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation 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PubStatus="accepted"><Year>2011</Year><Month>10</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>12</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>12</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>4</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22133602</ArticleId><ArticleId IdType="pii">S0960-8524(11)01508-2</ArticleId><ArticleId IdType="doi">10.1016/j.biortech.2011.10.042</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Jiang, Xinglin" sort="Jiang, Xinglin" uniqKey="Jiang X" first="Xinglin" last="Jiang">Xinglin Jiang</name><name sortKey="Liu, Wei" sort="Liu, Wei" uniqKey="Liu W" first="Wei" last="Liu">Wei Liu</name><name sortKey="Sun, Yuanzhang" sort="Sun, Yuanzhang" uniqKey="Sun Y" first="Yuanzhang" last="Sun">Yuanzhang Sun</name><name sortKey="Xian, Mo" sort="Xian, Mo" uniqKey="Xian M" first="Mo" last="Xian">Mo Xian</name><name sortKey="Zhao, Guang" sort="Zhao, Guang" uniqKey="Zhao G" first="Guang" last="Zhao">Guang Zhao</name><name sortKey="Zheng, Yanning" sort="Zheng, Yanning" uniqKey="Zheng Y" first="Yanning" last="Zheng">Yanning Zheng</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Yang, Jianming" sort="Yang, Jianming" uniqKey="Yang J" first="Jianming" last="Yang">Jianming Yang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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